ANTIBIOTIC PROPHYLAXIS AND CLOACAL CARRIAGE OF RESISTANT ZOONOTIC BACTERIA IN COMMERCIALLY-BRED POULTRY

Authors

  • Reuben Essel Arhin Accra Technical University
  • Henry Kwadwo Hackman Accra Technical University
  • Alhassan Sa-eed Accra Technical University

DOI:

https://doi.org/10.47672/ejah.424

Keywords:

Antibiotics, prophylactics, zoonotic, poultry, resistant

Abstract

Purpose: The aim of this study was to determine the relationship between the use of antibiotic prophylactics in commercial poultry breeding and cloacal carriage of antibiotic-resistant zoonotic bacteria.

Methodology: Biodata was collected on poultry from 11 selected farms.  Cloacal swabs were collected from 10 birds from each farm for culture, isolation and biochemical identification of bacteria isolates. Antibiotic susceptibility of 96 Enterobacteriaceae and 24 Staphylococcus aureus isolates were determined by disk diffusion.

Findings: Antibiotics prophylaxis administered to the birds were chloramphenicol (40/110), penicillin (20/110), doxycycline (20/110), gentamicin (10/110), neomycin (10/100) and a combination of chloramphenicol, ampicillin, penicillin and cloxacillin (10/100).  These were administered either weekly (90/110), every 3 days (10/110) or monthly (10/100). Two hundred and fifty six (256) different bacteria isolates were recovered. These were Escherichia coli (31.6%), Staphylococcus aureus (14.5%), Staphylococcus epidermidis (12.1%), Proteus sp. (12.1%), Citrobacter sp. (9%), Proteus vulgaris (5.1%), Salmonella enterica (4.7%), Citrobacter koseri (4.3%), Klebsiella sp. (2.8%), Klebsiella pneumoniae (2.3%), Shigella sp. (2.3%), Enterobacter sp. (0.8%) and Klebsiella oxytoca (0.4%). Of 96 Enterobacteriaceae, 60 (63%) were multidrug resistant. Enterobacteriaceae were resistant to ampicillin (54%), tetracycline (52%), cotrimoxazole (54%), gentamicin (22%), cefuroxime (44%), vancomycin (19%), chloramphenicol (39%), ceftriaxone (29%), cefotaxime (71%), ciprofloxacicn (21%), amikacin (10%) and meropenem (23%). Of 24 Staphylococcus aureus, 17 (71%) were multidrug resistant. Staphylococcus aureus were resistant to ampicillin (89%), cotrimoxazole (59%), gentamicin (24%), vancomycin (67%), ciprofloxacin (18%), meropenem (33%), tetracycline (85%), cloxacillin (100%), penicillin (81%), erythromycin (71%), cefuroxime (43%) and augmentin (45%). Doxycycline-prophylaxis was significantly related (0.001) to tetracycline-nonsusceptible isolates but chloramphenicol-prophylaxis and penicillin-prophylaxis were not significantly related (<0.05) to resistance to their corresponding antibiotics.

Unique contribution to theory, practice and policy: Occurrence of multidrug resistant zoonotic bacteria was high as was the frequency of administering antibiotic prophylactics. Amikacin was the most effective antibiotic against Enterobacteriaceae whereas gentamicin and ciprofloxacin were the most effective against both Enterobacteriaceae and Staphylococcus aureus. To safeguard high-priority class antibiotics regulations to adhere to the WHO statement on the use of antibiotics in animal husbandry should be enforced. Alternative measures should also be applied to reduce dependence on antibiotics in poultry farming

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Author Biographies

Reuben Essel Arhin, Accra Technical University

 

Lecturer, Faculty of Applied Sciences

 

 

Henry Kwadwo Hackman, Accra Technical University

Senior Lecturer, Faculty of Applied Sciences

 

Alhassan Sa-eed, Accra Technical University

Principal Laboratory Technologist, Faculty of Applied Sciences

 

References

gyare, C., Boamah, V. E., Zumbi, C.N., & Osei, F.B. (2018). Antibiotic use in poultry production

and its effects on bacterial resistance. Intechopen. DOI:10.5772/intechopen.79371. Retrieved from http://dx.doi.org/10.5772/intechopen.79371

Akond, M.A., Alam, S., Hassan, S.M.R., & Shirin, M. (2009). Antibiotic resistance of Escherichia

coli isolated from poultry and poultry environment of Bangladesh. Internet Journal of Food Safety. 11, 19-23. Retrieved from https://www.researchgate.net/publication/26624026

Argudín, M.A., Deplano, A., Meghraoui, A., Dodmont, M., Heinrichs, A., Denis O., Nonhoff,

C., & Roisin, S. (2017). Bacteria from animals as a pool of antimicrobial resistance genes. Antibiotics, 6(2), 12. Retrieved from https://doi.org/10.3390/antibiotics6020012

Boneca, I.G., & Chiosis, G. (2003). Vancomycin resistance: occurrence, mechanisms and

strategies to combat it. Expert Opinion on Therapeutic Targets. 7(3), 311-328. Retrieved from http://www.ncbi.nlm.nih.gov/m/pubmed/12783569

Bounar-Kechih, S., Hamdi, M.T., Aggad, H., Meguenni, N., & Cantekin, Z. (2018).

Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria. Veterinary Medicine International. Volume 2018, Article ID 4636121, 5 pages. Retrieved from https://doi.org/10.1155/2018/4636121

Bengtsson-Palme, J, Kristiansson, E., & Larsson, D.G.J. (2017). Environmental factors

influencing the development and spread of antibiotic resistance. FEMS Microbiology Reviews. fux053, 42, 68-80. doi: 10.1093/femsre/fux053

Bush, K. (2010). Alarming betalactamase-mediated-resistance in multidrug-resistant Enterobacteriaceae. Current Opinion in Microbiology, 13(5), 558-564. Retrieved from https://www.sciencedirect.com/science/article/abs/pii/S1369527410001335

Bushan, C., Khurana, A, Sinha, R. & Nagaraju, M. (2017). Antibiotic resistance in poultry

environment: Spread of resistance from poultry farms to agricultural fields. New Delhi: Center for Science and Environment. Retrieved from

http://www.indiaenvironmentportal.org.in/files/file/report-antibiotic-resistance-poultry-environment.pdf

Clavijo, V. & Flórez, M.J.V. (2017). The gastrointestinal microbiome and its association with the

control of pathogens in broiler chicken production: A review. Gastrointestinal Microbiome in Poultry. Oxford University Press. Retrieved from

https://academic.oup.com/ps/article-abstract/97/3/1006/4739542

Dale E., & Brown, C. (2013). Zoonotic Diseases from Poultry. Brazilian Journal of Veterinary

Pathology. 6(2), 76-82. Retrieved from

https://pdfs.semanticscholar.org/5c9a/cc1b5b49b8856b117549dd15447b1629e56e.pdf

Duong, A. (2015). 6 factors that have caused antibiotic resistance. InfectionControl.tips

Munang'andu, M.H., Kabilika, S.H., Chibomba, O., Munyeme, M., & Muuka, G.M. (2012).

Bacteria isolations from broiler and layer chicks in Zambia. Journal of Pathogens. Volume 2012, Article ID 520564, 6 pages. doi:10.1155/2012/520564

Munita, J.M. & Arias, C.A. (2016). Mechanisms of antibiotic resistance. Microbiology

Spectrum. 2; 4(2): 10.1128/microbiolspec.VMBF-0016-2015. doi: 10.1128/microbiolspec.VMBF-0016-2015

Mwambete, K.D., & Stephen, W.S. (2015). Antimicrobial resistance profiles of bacteria isolated

from chicken droppings in Dar Es Salaam. International Journal of Pharmacy and Pharmaceutical Sciences. 7(9), 268-271. Retrieved from

https://pdfs.semanticscholar.org/a0fd/a1c45f0091b5223b4151e770c99adf426c3d.pdf

Nhung, N.T., Chansiripornchai, N., Juan, J., & Carrique-Mas, J.J. (2017). Antimicrobial

resistance in bacterial poultry pathogens: A review. Frontiers in Verterinary Science. doi:10.3389/fvets.2017.00126

Otalu, O.J., Junaidu, K., Chukwudi, O.K., & Jariath, U.V. (2011). Multi-drug resistant coagulase

positive Staphylococcus aureus from live and slaughtered chicken in Zaria, Nigeria. International Journal of Poultry Science.10 (11) 871-875. Retrieved from http://docsdrive.com/pdfs/ansinet/ijps/2011/871-875.pdf

Philips, I (1983). Environmental factors contributing to antibiotic resistance. Infection Control.

(6), 448-451. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/6558025

Rasmussen, M.M, Opintan, J. A., Frimodt-Møller, N., & Styrishave, B. (2015). Beta-lactamase

producing Escherichia coli isolates in imported and locally produced chicken meat from Ghana. Plos One. doi:10.1371/journal.pone.0139706

Robert, F. (2017). Superantibiotic is 25,000 times more potent than its predecessor. Science.

Retrieved from

https://www.sciencemag.org/news/2017/05/superantibiotic-25000-times-more-potent-its-predecessor

Ryan, C. (2018). EU bans prophylactic use of antibiotics in farming from 2022. News, Vet and

Medication. Retrieved October 17th, 2019, from

http://www.poultrynews.co.uk/news/eu-bans-prophylactic-use-of-antibiotics-in-farming-

from-2022.html

Scholar, E. (2017). Practical antimicrobial therapeutics in veterinary medicine. sciencedirect.com/topics/agricultural-and-biological-sciences/tetracycline

Shecho, M., Thomas, N., Kemal, J., & Muktar, Y. (2017). Cloacael carriage and multidrug

resistance Escherichia coli O157:H7 from poultry farms, Eastern Ethiopia. Journal of Verterinary Medicine. Article ID 8264583, 9 pages. Retrieved from http://dx.doi.org/10.1155/2017/8264583

Siddiqui, M.A., Khan, L.A., Suradkar, U.S., Mendhe, M.S., Rindhe, S.N. & Sirsat, P.R. (2008).

Bacterial isolation and their antibiogram from non-specific infection in poultry of Marathwada region. Veterinary World, 1(2), 52-53. Retrieved from

http://agris.fao.org/agris-search/search.do?recordID=DJ2012059636

Zaman, S., Hussain, M.W., Nye, R., Mehta, V., Mamun, K.T., & Hossain, N. (2017) A review on

antibiotic resistance: alarm bells are ringing. Cureus. 9(6), e1403. doi 10.7759/cureus.1403

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Published

2019-11-05

How to Cite

Arhin, R. E., Hackman, H. K., & Sa-eed, A. (2019). ANTIBIOTIC PROPHYLAXIS AND CLOACAL CARRIAGE OF RESISTANT ZOONOTIC BACTERIA IN COMMERCIALLY-BRED POULTRY. European Journal of Animal Health, 1(1), 1–13. https://doi.org/10.47672/ejah.424

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