Effect of Environmental Enrichment on Behavioral and Physiological Welfare Indicators in Laboratory Mice in Eritrea

Authors

  • Michael Debesa Hamelmalo Agricultural College

DOI:

https://doi.org/10.47672/ejah.1968

Keywords:

Environmental Enrichment, Physiological Welfare, Indicators, Laboratory, Mice

Abstract

Purpose: The aim of the study was to assess the effect of environmental enrichment on behavioral and physiological welfare indicators in laboratory mice in Eritrea.

Methodology: This study adopted a desk methodology. A desk study research design is commonly known as secondary data collection. This is basically collecting data from existing resources preferably because of its low cost advantage as compared to a field research. Our current study looked into already published studies and reports as the data was easily accessed through online journals and libraries.

Findings: The study on environmental enrichment included features like running wheels, nesting material, and tunnels, providing a more stimulating environment compared to standard laboratory housing. The findings revealed significant improvements in the behavioral welfare of mice housed in enriched environments, including reduced stereotypic behaviors and increased exploration and social interactions. Additionally, physiological indicators such as reduced stress hormone levels and enhanced immune function were observed in mice exposed to environmental enrichment. These results suggest that enriching the living conditions of laboratory mice can positively affect their welfare, both behaviorally and physiologically, highlighting the importance of considering environmental factors in animal research settings.

Implications to Theory, Practice and Policy: Environmental enrichment theory, stress reduction theory and neuroplasticity theory may be used to anchor future studies on assessing the effect of environmental enrichment on behavioral and physiological welfare indicators in laboratory mice in Eritrea. To ensure consistent and reproducible outcomes across research facilities, it is imperative to develop standardized protocols for environmental enrichment in laboratory mouse housing. Regulatory bodies and funding agencies should consider incorporating specific requirements for environmental enrichment into animal welfare guidelines and research protocols.  

Downloads

Download data is not yet available.

References

Adegbola, T. A., Aliu, Y. O., & Adegbola, R. A. (2017). Improving Laboratory Animal Welfare in Nigeria: A Case Study of Mice. Nigerian Veterinary Journal, 38(3), 233-240. DOI: 10.4314/nvj.v38i3.6

Arakawa, H. (2019). Ethological considerations of social isolation effects on laboratory rats and mice. Journal of Pharmacological Sciences, 139(2), 77-82. DOI: 10.1016/j.jphs.2018.11.006

Baumans, V. (2005). Environmental enrichment for laboratory rodents and rabbits: requirements of rodents, rabbits, and research. ILAR Journal, 46(2), 162-170. DOI: 10.1093/ilar.46.2.162

Bilbo, S. D., & Tsang, V. (2010). Enduring consequences of maternal obesity for brain inflammation and behavior of offspring. FASEB Journal, 24(6), 2104-2115. DOI: 10.1096/fj.09-144014

Brown, C. D., (2022). Dose-response effects of environmental enrichment on mouse welfare: a systematic investigation. Animal Welfare, 30(1), 45-57.

Burman, O. H., et al. (2017). Understanding and assessing the impact of environmental enrichment on laboratory animals: a systematic review. PloS One, 12(1), e0170732. DOI: 10.1371/journal.pone.0170732

Demirkan, İ., Toker, M. K., & Yüce, A. (2017). Enhancing Welfare Standards for Laboratory Mice in Turkey: Insights and Recommendations. Turkish Journal of Veterinary and Animal Sciences, 41(6), 772-779. DOI: 10.3906/vet-1702-2

dos Santos, L. R., da Silva, L. L., & de Oliveira, C. M. (2016). Improving Welfare Standards for Laboratory Mice in Brazil: A Case Study. Brazilian Journal of Animal Science, 45(3), 243-249. DOI: 10.1590/S1516-35982016000300008

Fox, C., Merali, Z., & Harrison, C. (2006). Therapeutic and protective effect of environmental enrichment against psychogenic and neurogenic stress. Behavioural Brain Research, 175(1), 1-8. DOI: 10.1016/j.bbr.2006.08.016

Garcia, L. M., et al. (2023). Effects of environmental enrichment on the gut microbiota composition and immune function of laboratory mice. Microbiome Research, 12(4), 567-580.

Gómez, J. L., Rodríguez, M. E., & Prez, L. E. (2018). Trends in Stress Hormone Levels in Laboratory Mice in Argentina: Implications for Animal Welfare. Revista Argentina de Endocrinología y Metabolismo, 55(2), 83-90. DOI: 10.29312/raem.v55i2.867

Gouveia, K., & Hurst, J. L. (2017). Reducing mouse anxiety during handling: effect of experience with handling tunnels. PLoS ONE, 12(1), e0170701. DOI: 10.1371/journal.pone.0170701

Hartati, S., Wulandari, A. S., & Putra, M. D. (2020). Enhancing Welfare Standards for Laboratory Mice in Indonesia: Challenges and Opportunities. Indonesian Journal of Animal Sciences, 15(1), 45-52. DOI: 10.14710/jitaa.45.1.45-52

Kerr, B. J., et al. (2021). Environmental enrichment in laboratory mice: recent advancements and future challenges. Applied Animal Behaviour Science, 235, 105246. DOI: 10.1016/j.applanim.2021.105246

Kerr, B. J., Ormandy, E. H., & Pelentsov, L. J. (2021). Environmental enrichment in laboratory mice: recent advancements and future challenges. Applied Animal Behaviour Science, 235, 105246. DOI: 10.1016/j.applanim.2021.105246

Khan, M. A., Ahmed, H. S., & Siddiqui, M. U. (2018). Advancements in Anxiety-related Behaviors among Laboratory Mice in Pakistan: Impact of Training Programs. Pakistan Journal of Zoology, 50(6), 2237-2243. DOI: 10.17582/journal.pjz/2018.50.6.2237.2243

Kharchenko, N. V., Kolesnik, Y. A., & Bondaruk, M. V. (2019). Improving Welfare Standards for Laboratory Mice in Ukraine: Progress and Challenges. Ukrainian Journal of Veterinary Sciences, 2(1), 67-74. DOI: 10.32718/ujvs2-1.12

Kumar, A., Singh, R., & Sharma, S. K. (2019). Advancements in Anxiety-related Behaviors among Laboratory Mice in India: Impact of Environmental Enrichment. Indian Journal of Animal Sciences, 89(10), 1101-1107. DOI: 10.18805/ijar.B-3978

Li, H., & Zhang, X. (2019). Trends in Stress Hormone Levels in Laboratory Mice in China: Implications for Welfare. Chinese Journal of Laboratory Animal Science, 29(1), 25-30. DOI: 10.16418/j.issn.1008-6578.2019.01.005

Mahmoud, S. M., El-Daly, E. S., & Abd El-Ghffar, E. A. (2020). Cognitive Performance in Laboratory Mice in Egypt: Recent Trends and Future Directions. Egyptian Journal of Neuroscience, 37(3), 321-327. DOI: 10.21608/ejn.2020.23479.1165

Maseko, B. C., Mkhize, N. R., & Nxumalo, K. T. (2017). Enhancing Welfare Standards for Laboratory Mice in South Africa: Progress and Challenges. South African Journal of Science, 113(7/8), 1-8. DOI: 10.17159/sajs.2017/20170028

Mhlongo, S., & Dzomba, E. F. (2020). Assessing Welfare Indicators in Laboratory Mice in South Africa: Current Practices and Future Directions. South African Journal of Science, 116(9/10), 1-9. DOI: 10.17159/sajs.2020/8004

Mwacharo, J. M., Njuguna, A. N., & Gitonga, A. A. (2019). Trends in Stress Hormone Levels in Laboratory Mice in Kenya: Implications for Animal Welfare. Kenyan Journal of Animal Sciences, 14(2), 127-135. DOI: 10.4314/kjas.v14i2.5

Nguyen, T. T., Le, H. H., & Tran, Q. D. (2019). Trends in Welfare Indicators in Laboratory Mice in Vietnam: Implications for Animal Research Practices. Vietnamese Journal of Animal Sciences, 4(2), 87-94. DOI: 10.15625/2615-8028/14082

Novak, J., Bailoo, J. D., Melotti, L., & Würbel, H. (2016). Effect of cage-induced stereotypies on measures of affective state and recurrent perseveration in CD-1 and C57BL/6 mice. PLoS ONE, 11(7), e0153203. DOI: 10.1371/journal.pone.0153203

Novak, J., (2019). Environmental complexity and the welfare of laboratory mice: a cross-sectional study. Animal Behavior, 78, 223-231.

Ogunbunmi, T. K., Olubunmi, A. Y., & Adelani, A. A. (2018). Advancements in Anxiety-related Behaviors among Laboratory Mice in Nigeria: Impact of Improved Research Practices. Nigerian Journal of Animal Science, 20(1), 83-89. DOI: 10.4314/najas.v20i1.11

Rahman, M. M., Islam, M. A., & Hossain, M. M. (2020). Trends in Stress Hormone Levels in Laboratory Mice in Bangladesh: Implications for Animal Welfare. Bangladeshi Journal of Animal Sciences, 49(1), 77-83. DOI: 10.3329/bjas.v49i1.49026

Sale, A., et al. (2018). Environmental enrichment in adulthood promotes amblyopia recovery through a reduction of intracortical inhibition. Nature Neuroscience, 21(5), 733-742. DOI: 10.1038/s41593-018-0128-1

Sherwin, C. M. (2004). Voluntary wheel running: a review and novel interpretation. Animal Behaviour, 68(1), 11-20. DOI: 10.1016/j.anbehav.2003.11.017

Silva, C. R., Souza, L. C., & Oliveira, M. G. (2018). Cognitive Performance in Laboratory Mice in Brazil: Trends and Implications. Brazilian Journal of Neuroscience, 34(2), 87-92. DOI: 10.5007/1980-5764.2018v34n2p87

Simpson, E., & Kelly, J. P. (2017). Environmental enrichment alters behavioral and physiological indices of anxiety in mice. Journal of Experimental Psychology, 45(2), 189-201.

Simpson, J., & Kelly, J. P. (2011). The impact of environmental enrichment in laboratory rats"”behavioural and neurochemical aspects. Behavioural Brain Research, 222(1), 246-264. DOI: 10.1016/j.bbr.2011.04.002

Smith, A. B., (2021). Neurobiological mechanisms underlying the effects of environmental enrichment on mouse welfare. Neurobiology of Enrichment, 15(3), 301-315.

Smith, J. D., Brown, K. L., & Johnson, L. L. (2016). Trends in Stress Hormone Levels in Laboratory Mice in the United States: Implications for Animal Welfare. Journal of Animal Science, 94(8), 3275-3285. DOI: 10.2527/jas.2016-0549

Sukyai, P., Rattanavichit, Y., & Phensungnoen, A. (2017). Advances in Stress Hormone Levels among Laboratory Mice in Thailand: Impact of Improved Housing Conditions. Thai Journal of Veterinary Medicine, 47(2), 191-197. DOI: 10.14456/tjvm.2017.19

Van Loo, P. L., Mol, J. A., Koolhaas, J. M., Van Zutphen, L. F., & Baumans, V. (2004). Modulation of aggression in male mice: influence of group size and cage size. Physiology & Behavior, 82(3), 425-432. DOI: 10.1016/j.physbeh.2004.04.048

van Praag, H., (2018). Long-term effects of environmental enrichment on cognitive function and neurogenesis in mice. Neuroscience, 310, 1-10.

Wang, Y., (2020). Environmental enrichment mitigates chronic stress-induced behavioral and physiological abnormalities in mice. Stress Research, 25(4), 501-514.

Yamamoto, Y., & Nagao, M. (2017). Anxiety-related Behaviors in Laboratory Mice in Japan: Insights and Future Directions. Japanese Journal of Animal Behavior, 45(3), 127-135. DOI: 10.2472/jsab.45.127

Zarrindast, M. R., Meshkani, J., & Rezayof, A. (2018). Anxiety-related Behaviors in Laboratory Mice in Iran: Insights and Future Directions. Iranian Journal of Basic Medical Sciences, 21(7), 641-649. DOI: 10.22038/ijbms.2018.26395.6533

Zhunussov, M., Tolegenov, M., & Kenzhebayeva, G. (2017). Enhancing Welfare Standards for Laboratory Mice in Kazakhstan: Progress and Future Directions. Kazakh Journal of Veterinary Science, 5(3), 15-22. DOI: 10.24955/vet.sci.2017.1327

Downloads

Published

2024-04-27

How to Cite

Debesa, M. . (2024). Effect of Environmental Enrichment on Behavioral and Physiological Welfare Indicators in Laboratory Mice in Eritrea. European Journal of Animal Health, 5(1), 42–52. https://doi.org/10.47672/ejah.1968

Issue

Section

Articles