PROXIMATE AND PHYTOCHEMICAL ANALYSIS OF HEALTHY AND INFECTED MAIZE GRAINS IN ANAMBRA STATE
DOI:
https://doi.org/10.47672/ejb.419Keywords:
Proximate analysis, phytochemical analysis, maize.Abstract
Four samples of stored maize grains were bought from Awka, Awkuzu, Onitsha and Nnewi. Direct and indirect methods of Fawole and Oso, 1998 were used to isolate the fungi responsible for the spoilage of post harvest maize grains after which four genera were isolated namely: Curvularia spp, Fusarium spp, Rhizopus spp and Aspergillus spp. They were identified using Barmette and Haunter, 1987 method of identification. The percentage of occurrence of the fungi from different samples shows that Aspergillus niger occured with a frequency of 31.5%, A. flavus with 31%, Rhizopus spp with 15.25%, Fusarium spp with 14.5% and the least Curvularia spp with 7.5%. AOAC method of 1999 was used to obtain the nutritional values of the maize grains. Good and spoilt maize grains were used in this research. Spoilt maize grains were used after four months of purchase. Anova were used to compare the nutritional values of both good and spoilt maize samples from the different locations. Six nutrients were obtained from both good and spoilt maize grains namely: crude protein, crude fibre, crude fat, moisture content, ash content and carbohydrate. The results differ in their values for both good and spoilt maize grains. The spoilt maize grains have little protein, fibre and fat and more of ash and carbohydrate. Further research was made on the phytochemical contents of maize and seven metabolites were obtained mamely: oxalate, phytate, tannin, phenol, alkaloid, flavonoid and glycoside. In order to reduce post-harvest grain loss of maize, periodical training of farmers on proper storage facilities are necessaryDownloads
References
Barmette, H.L. and Hunter, B.B. (1987). Illustrated genera of imperfect fungi 2nd edition.Burgress publishing camp. Pp:225.
Barros, L.D, Ferreira, M.J, Queiros, B.A, Ferreira,I.C. and Batista, P. (2007).Total phenols,ascorbic acid, á-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chemistry.103:1314-419.
Campbell, J. F. Arthur, F.H. and Mullen, M.A. (2004). Insect management in food processing facilities.Advances in Food and Nutrition Research 48: 239-295.
Devereau, A. D, Myhara,M.B. and Anderson, C.H. (2002). Chapter 3: Physical factors in post-harvest quality. Crop Post-Harvest: Science and Technology: Principles and Practice, Volume 1. 62-92. eds. Ames, Iowa: Blackwell Science Ltd.
Dhawale, S.U and LaMaster ,A.O (2003).Microbiology laboratory manual. The mcHillcompany.inc.USA (5):209-229.
Egal, S.O, Hounsa, Y.K, Gong, P.C, Turner, C. P,Wild, A. J, Hall, K.and Cardwell, K.F. (2005). Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa. International Journal of Food Microbiology 104(2): 215- 224.
Ekechukwua, O. V. and Norton. B.B (1999). Review of solar-energy drying systems II: an overview of solar drying technology. Energy Conversion and Management 40(6): 615-655.
FAO. (2006). Maize: international market profile. Grains team food and agriculture organization of the United Nations economic and social department trade and markets division. p94
Fawole, B.A. and Oso, B.A. (1988). The journal of food technology in Africa, Vol.6, no.3, pp87-89.
Golob, P.I, Kutukwa, A,T, Devereau, R.N, Bartosik, E.Y and Rodriguez. J.C. (2004). Chapter two: Maize. Crop Post-Harvest: Science and Technology, Volume 2. R. Hodges, and G. Farrell, eds. Ames, Iowa.Blackwell Publishing Ltd, 2245-2257.
Ikenie, J.E, Amusan, N.A. and Obtaolu, V.O. (2002). Nutrient composition and weight evaluation of some newly developed maize varieties in Nigeria. J. Food Techn. in Africa. Vol. 7; Pp 27-29
Jian, F, and Jayas, D.S. (2012). The ecosystem approach to grain storage. Agricultural Research 1(2): 148-156.
Lee, S. (1999). Low-temperature damp corn storage with and without chemical preservatives. Doctoral (PhD) dissertation.The University of Guelph.
Matilda, A, Rune, N, Oyvind, L. and Einar, L. (1993). Effect of Processing (Sprouting and or Fermentation) on Sorghum and Maize.1: Proximate Composition, Minerals and Fatty Acids. Food chemistry. 46:351-353
Montross, J. E, Montross, M.D. and Bakker-Arkema, F.W. (1999). Part 1.4 Grain storage 46-59.eds. CIGR Hand Book of Agricultural Engineering.Volume IV. Agro-Processing Engineering. St. Joseph, Michigan, USA.
Osagie, A.U. (1998). Anti-nutritional Factors. In: Nutritional Quality of Plant Foods. Ambik Press Ltd, Benin City, Nigeria. Pp. 1-40; 221-244
Oyekale, K. O, Daniel, M. O, Ajala, C.H. and Sanni, L.O. (2012). Potential longevity of maize grains under storage in humid tropical seed stores. Nature and Science 10(8): 114-124.
Samir, T, Kraszaewsk, A.W, Nelson, S.O. (1998).Non destructive microwave Characterization for Determining the bulk density and moisture Contents of Shelled Gru. Sci. Technol., 9:1548-1556.
Sasaki, N. (1995). Maize Market Liberalization, Seasonal Price, and Private Sector Storage. In Processing of the conference on Towards 200; Improving Agricultural Performance, EgertonUni, Policy Analysis Matrix, Nairobi.
Siddhuraju , P. and Manian S. (2007). The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotylomauniflorum (Lam.) Verdc.) grains. Food Chem105(3): 950-958.
Tuite, J.T. and Foster, G.H. (1979). Control of storage diseases of grain. Annual Review of Phytopathology 17(1)
Ujabadeniyi, A.O. and Adebolu, J.T (2005). The effect of processing method on nutritional properties of ogi produced from three maize varieties. J. Food, Agric and Environment. 3:108-109.
Yaouba, A, Tatsadjieu, N.L, Jazet, D.P. and Mbofung, C.M. (2012). Inhibition of fungal development in maize grains under storage condition by essential oils. International Journal of Biosciences 2 (6): 41-48.
Young and Greaves.(1940). Laboratory Handbook of Methods of Food Analysis, Leonard Hill, London. Pp. 217-273.
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